This nocturnal mammal is thought to be a solitary bat, which inhabits trees and feeds continuously during hours of darkness (2). In captivity, Pohle's fruit bat has fed on fruit such as bananas, guavas and plantains. Females seem to be the more active and the more vocal sex, emitting a high-pitched whistle throughout the night (2).
The available data indicate that most female Pohle's fruit bats are pregnant during August and September, but there may also be a second breeding period at the end of the year. A single young is born at a time (2).