Like other members of the genus Ctenomys, the southern tuco-tuco is herbivorous and feeds on grasses and shrubs. It eats both the leaves and roots of the plants (6).
The southern tuco-tuco is solitary and highly territorial, building large burrow systems in sand dunes (7). Further information on the southern tuco-tuco’s behavior is scarce; however, members of the genus Ctenomys are typically diurnal, alternating periods of activity and rest throughout the day (6).
Little is known about the courtship and mating of tuco-tucos, as these behaviours take place inside the burrows. The male is known to take an aggressive posture, and both the male and female probably exchange chemical or acoustic signals, but further details are unknown. The southern tuco-tuco is polygynous, meaning that a male may mate with several females (8).
The gestation period of the southern tuco-tuco is approximately 100 days. After this time, the female gives birth to between two and six pups, with lactation lasting around two months. The southern tuco-tuco has two reproductive periods per year (8).