The most significant threat to the survival of remaining maned wolf populations is habitat loss (8). The conversion of land to agriculture has drastically reduced the available habitat for the maned wolf, with the cerrado of Brazil being reduced to about 20 percent of its original extent (8). In addition maned wolves are often killed on highways, frequently on those which border protected areas. Indeed, road kills are responsible for the death of approximately half the annual production of pups in some reserves (8). Domestic dogs also pose a threat by transferring diseases, competing for food, and even killing the maned wolf (1).
Some local people attribute mystical qualities to several parts of the wolf's anatomy (eyes, skin, tail) and still hunt this threatened species in order to use these parts as ‘talisman' or for medicinal remedies (6). Occasionally, this wolf is hunted for sport (5), and, due to the wolf's threat to domestic poultry, farmers also hunt it as a pest (6). As its habitat is encroached upon by ever-expanding farms, the wolf is forced into increased proximity with people, exacerbating the already-existing conflict (6).
The maned wolf occurs in a number of protected areas across its range. Although protected by law in certain countries, with hunting prohibited in Brazil, Paraguay and Bolivia, law enforcement is often problematic. At present, there are no known conservation actions specific to the maned wolf, but there are broader attempts to protect parts of its habitat and reduce the impact of animal road kills in Brazil (1). Encouragingly, observations indicate that the maned wolf is able to colonize different habitats and that the species' range has altered in configuration in recent years rather than diminished (12). This has, however, led these wolves into areas of greater proximity and conflict with humans, and education programmes have therefore been started to dissuade farmers from shooting this rare species (2). As of 2003, 146 institutions reported a total of 431 maned wolves in captivity, including 208 males and 222 females (8). However, for unknown reasons, canids breed poorly in captivity. Research has therefore been conducted into behaviour affecting hormones, nutrition and stress in captivity, as well as the use of modern reproductive technologies to aid the process (10). Future studies need to focus on population surveys throughout the species' range, as well as research into how human encroachment and habitat loss is impacting this distinctive canid (1).