The little pied bat prefers roosting in tree hollows and caves, but will also live inside abandoned buildings (1) (4) (6) (8). Colonies of this species may number up to 50 individuals (1). The little pied bat hunts in vegetated areas, and is unlikely to be found flying in the open (3). During the night, this species forages around water for small flying insects, such as moths (2) (4).
Given the difficulty in observing this species, there is little specific information available on the biology and behaviour of the little pied bat. However, it is known to breed during the spring and summer and it has two young per litter, although the number of litters per year is unknown (2).