Although the Lami tuco-tuco is a relatively little-known species, its biology and life history is likely to be similar to that of other tuco-tucos (Ctenomys species).
Proficient burrowers, tuco-tucos excavate extensive underground networks of tunnels and chambers, which are typically never more than 30 centimetres below the surface (4). Heaps of soil at the surface, like molehills, mark the courses of the burrows (4). Although most species of tuco-tuco are solitary, with each adult occupying its own burrow, some species are known to live in groups in the same burrow system (8). Whether the Lami tuco-tuco is a solitary or gregarious species is not yet clear.
Tuco-tucos are herbivorous rodents, feeding solely on plant material. This includes roots and tubers (5) (8), which are probably pulled into the burrow from below ground (8), as well as stems and grasses, which the tuco-tuco has to venture above ground to collect (5). Tuco-tucos hoard food, storing it in chambers within the burrow system, and this food seems to provide all the water the tuco-tuco requires (4). Tuco-tucos are most active early in the morning and late in the afternoon, and spend the rest of the day in the burrow (4). Before venturing from the burrow, the tuco-tuco is able to check its surroundings for predators without exposing itself, as its eyes are almost level with the top of the head (8).
Female Lami tuco-tucos typically fall pregnant in the winter and give birth in the spring (3). The gestation period of tuco-tucos typically lasts 105 to 120 days, after which the female gives birth in a grass-lined chamber to up to seven young. The well-developed young are able to leave the nest and feed themselves shortly after birth. Tuco-tucos typically breed before they are a year old, but generally live no longer than three years (4).