Although little is known about the Javan tailless fruit bat, it is likely that, like other fruits bats, it is a nocturnal species with a frugivorous diet (2). The Javan tailless fruit bat probably spends the day roosting in trees in small groups or individually (1), where it hangs upside down by its feet (2). At dusk, this species will leave the roost and fly in search of fruit, which it locates using its sight and sense of smell. It crushes ripe fruit in its mouth, swallowing the juice and spitting out most of the pulp and seeds. Details of the Javan tailless fruit bats diet are not known, but other Old World fruit bats may also chew flowers to obtain nectar and juices (2). Due to the frugivorous diet of fruit bats, they play an important role in pollinating and dispersing the seeds of plants, many of which have great commercial value (3).
No information is available on the breeding biology of the Javan tailless fruit bat but, like other Old World fruit bats, it probably gives birth to a single young (2).