The Iowa Pleistocene snail retreats into the soil around October to hibernate, although this may occur earlier if it is particularly cold. The snails hibernate until March or April when the ice thaws (2).
Breeding season is confined to between March/April to August. The Iowa Pleistocene snail is hermaphroditic, as are most North American land snails, but they are not self-fertilising. They are reported to be able to both lay eggs and fertilise others. Iowa Pleistocene snails lay their eggs under logs, in rock crevices or in the soil. The gestation period is not known, but individuals have been observed to have several broods a year. The average clutch size is three, and hatching occurs about 28 days after the eggs are laid, commonly with a 90 percent hatch rate (2).
In captivity, Iowa Pleistocene snails required between two and two and a half years to reach sexual maturity. Juvenile snails are much more active than adult Iowa Pleistocene snails. They are typically the last to hibernate, and the first to emerge in the spring, but they are more susceptible to drying out than adults, and are less often seen outside the confines of a colony as a result (2).