The Comanche Springs pupfish can live for up to two years, although most only live for around one year (4). As a result of its short lifespan, this pupfish has no distinct breeding season and is capable of spawning year-round (9).
The male Comanche Springs pupfish establishes a territory which is usually in swift water and will contain an algal mat onto which a female can spawn (10). When competing for a mate, the male moves into an upstream position within its territory and defends the area until a female arrives (10). Large females produce more eggs than smaller females (11) and the eggs generally hatch after around five days (12). The male fiercely defends the eggs until they hatch (10).
Although some male Comanche Springs pupfishes maintain a territory and will reproduce when a female enters the area, others may use one of two alternative techniques to mate, known as 'satellite positioning' and 'sneak spawning'. Satellite positioning involves an average-sized male occupying the periphery of the territory of a larger male and attempting to mate with females when they enter the territory. Sneak spawning is used by small males who have a similar appearance to the females. The larger males may not notice that the smaller individual is actually a male, allowing the smaller male to reproduce with the female without being detected and contested by larger conspecifics (13).
The diet of the Comanche Springs pupfish consists of algae and small invertebrates (4).